Introduction

In this chapter we describe MultiPLX-a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from http://bioinfo.ut.ee/?page_id=167. Graphical web-based interface to most functions of MultiPLX is available at http://bioinfo.ut.ee/multiplx/.

Publications

  1. MultiPLX: automatic grouping and evaluation of PCR primers.
    Cite this
    Kaplinski L, Remm M, 2015-01-01 - Methods in molecular biology (Clifton, N.J.)
  2. MultiPLX: automatic grouping and evaluation of PCR primers.
    Cite this
    Kaplinski L, Remm M, 2007-01-01 - Methods in molecular biology (Clifton, N.J.)
  3. MultiPLX: automatic grouping and evaluation of PCR primers.
    Cite this
    Kaplinski L, Andreson R, Puurand T, Remm M, 2005-04-01 - Bioinformatics (Oxford, England)

Credits

  1. Lauris Kaplinski
    Developer

    Department of Bioinformatics, University of Tartu, Estonia

  2. Maido Remm
    Investigator

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Summary
AccessionBT000714
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesC++
User InterfaceTerminal Command Line
Download Count0
Submitted ByMaido Remm