| Accession |
PRJCA001063 |
| Title |
Single-Cell RNA-seq Highlights Intra-tumoral Heterogeneity and Malignant Progression in Pancreatic Ductal Adenocarcinoma |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
Herein, we implemented the single-cell RNA-seq to determine the transcriptomes of over 50,000 individual pancreatic cells from 24 primary PDAC tumors and 11 normal pancreas. We detected two ductal subtypes representing nonmalignant and malignant ductal cells in PDAC. The malignant cells show high CNV and heterogeneity within and between tumors, in which specific proliferation and metastatic sub-malignant cells present in PDAC patients. Computational cell trajectory analysis revealed that multiple pathways and TFs dynamic expressed during the malignant, proliferation and metastasis progress. By integrating single-cell transcriptomes with bulk expression prfiles for PDAC, we established the association of malignant ductal cells and T cell signals with clinical pathological features. |
| Sample scope |
Single cell |
| Release date |
2019-07-09 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 31273297
|
Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.
|
Cell Research
|
10.1038/s41422-019-0195-y
|
2019
|
| 33819739
|
Single-cell RNA-seq reveals dynamic change in tumor microenvironment during pancreatic ductal adenocarcinoma malignant progression
|
EBioMedicine
|
10.1016/j.ebiom.2021.103315
|
2021
|
| 35319989
|
Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
|
Science Advances
|
10.1126/sciadv.abh4050
|
2022
|
| 33544846
|
SPOTlight: seeded NMF regression to deconvolute spatial transcriptomics spots with single-cell transcriptomes
|
Nucleic Acids Research
|
10.1093/nar/gkab043
|
2021
|
| 36864399
|
Integrated transcriptional analysis reveals macrophage heterogeneity and macrophage-tumor cell interactions in the progression of pancreatic ductal adenocarcinoma
|
BMC Cancer
|
10.1186/s12885-023-10675-y
|
2023
|
| 38631549
|
Stromal Rigidity Stress Accelerates Pancreatic Intraepithelial Neoplasia Progression and Chromosomal Instability via Nuclear Protein Tyrosine Kinase 2 Localization
|
The American Journal of Pathology
|
10.1016/j.ajpath.2024.02.023
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
31625016
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81672443
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81773292
|
|
| Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine
|
|
2017I2M1001
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key R&D Program of China
|
2018YFA0109700
|
|
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDA16010501
|
|
|
|
| Submitter |
sun
baofa (sunbf@big.ac.cn)
|
| Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
| Submission date |
2018-10-12 |
