Accession PRJCA001063
Title Single-Cell RNA-seq Highlights Intra-tumoral Heterogeneity and Malignant Progression in Pancreatic Ductal Adenocarcinoma
Relevance Medical
Data types Transcriptome or Gene expression
Organisms Homo sapiens
Description Herein, we implemented the single-cell RNA-seq to determine the transcriptomes of over 50,000 individual pancreatic cells from 24 primary PDAC tumors and 11 normal pancreas. We detected two ductal subtypes representing nonmalignant and malignant ductal cells in PDAC. The malignant cells show high CNV and heterogeneity within and between tumors, in which specific proliferation and metastatic sub-malignant cells present in PDAC patients. Computational cell trajectory analysis revealed that multiple pathways and TFs dynamic expressed during the malignant, proliferation and metastasis progress. By integrating single-cell transcriptomes with bulk expression prfiles for PDAC, we established the association of malignant ductal cells and T cell signals with clinical pathological features.
Sample scope Single cell
Release date 2019-07-09
Publication
PubMed ID Article title Journal name DOI Year
31273297 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. Cell Research 10.1038/s41422-019-0195-y 2019
33819739 Single-cell RNA-seq reveals dynamic change in tumor microenvironment during pancreatic ductal adenocarcinoma malignant progression EBioMedicine 10.1016/j.ebiom.2021.103315 2021
35319989 Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8 Science Advances 10.1126/sciadv.abh4050 2022
33544846 SPOTlight: seeded NMF regression to deconvolute spatial transcriptomics spots with single-cell transcriptomes Nucleic Acids Research 10.1093/nar/gkab043 2021
36864399 Integrated transcriptional analysis reveals macrophage heterogeneity and macrophage-tumor cell interactions in the progression of pancreatic ductal adenocarcinoma BMC Cancer 10.1186/s12885-023-10675-y 2023
38631549 Stromal Rigidity Stress Accelerates Pancreatic Intraepithelial Neoplasia Progression and Chromosomal Instability via Nuclear Protein Tyrosine Kinase 2 Localization The American Journal of Pathology 10.1016/j.ajpath.2024.02.023 2024
Elevated SAMD3 expression in T cells predicts improved survival in pancreatic ductal adenocarcinoma patients Cancer Immunology, Immunotherapy 10.1007/s00262-025-03948-x 2025
40215177 Dissecting FAP+ Cell Diversity in Pancreatic Cancer Uncovers an Interferon-Response Subtype of Cancer-Associated Fibroblasts with Tumor-Restraining Properties Cancer Research 10.1158/0008-5472.CAN-23-3252 2025
39472297 ARP2/3 complex affects myofibroblast differentiation and migration in pancreatic ductal adenocarcinoma International Journal of Cancer 10.1002/ijc.35246 2025
39811640 Gene expression dynamics in fibroblasts during early-stage murine pancreatic carcinogenesis iScience 10.1016/j.isci.2024.111572 2025
39757146 Single cell and spatial analysis of immune-hot and immune-cold tumours identifies fibroblast subtypes associated with distinct immunological niches and positive immunotherapy response Molecular Cancer 10.1186/s12943-024-02191-9 2025
41027967 Diabetes reshapes pancreatic cancer-associated endothelial niche by accelerating senescence Nature Communications 10.1038/s41467-025-63801-8 2025
41350522 Targeting TNK2/ACK1 reverses the immunosuppressive tumor microenvironment and synergizes with immunochemotherapy in pancreatic cancer Nature Communications 10.1038/s41467-025-67197-3 2025
41372157 SIDISH integrates single-cell and bulk transcriptomics to identify high-risk cells and guide precision therapeutics through in silico perturbation Nature Communications 10.1038/s41467-025-66162-4 2025
39747201 Integrative multi-omics analysis reveals the role of toll-like receptor signaling in pancreatic cancer Scientific Reports 10.1038/s41598-024-84062-3 2025
BIGH3 is a mediator of TGFβ-Induced collagen formation in fibrosis and pancreatic cancer and a potential therapeutic target Scientific Reports 10.1038/s41598-025-24463-0 2025
40083936 The TMBIM1-YBX1 axis orchestrates MDSC recruitment and immunosuppressive microenvironment in pancreatic cancer Theranostics 10.7150/thno.111180 2025
39526455 Establishing a new-onset diabetes-related metabolism signature for predicting the prognosis and immune landscape in pancreatic cancer Carcinogenesis 10.1093/carcin/bgae072 2025
One-carbon metabolic reprogramming and its relationship with tumor-infiltrating lymphocytes and Immune checkpoint in Pancreatic cancer Research Square 10.21203/rs.3.rs-6404670/v1 2025
39847195 Single-cell RNA-seq analysis reveals microenvironmental infiltration of myeloid cells and pancreatic prognostic markers in PDAC Discover Oncology 10.1007/s12672-025-01830-x 2025
41306373 IL1RA+ Myeloid-derived Suppressor Cells Activate Epithelial-mesenchymal Transition to Facilitate Lymphatic and Hepatic Metastasis in Pancreatic Ductal Carcinoma Journal of Clinical and Translational Hepatology 10.14218/JCTH.2025.00416 2025
40923379 Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma Advanced Science 10.1002/advs.202511726 2025
41159730 Single-cell multi-omics and machine learning for dissecting stemness in cancer Briefings in Bioinformatics 10.1093/bib/bbaf566 2025
41465457 Elucidating Pancreatic Ductal Adenocarcinoma Carcinogenesis at Single-Cell Resolution and Identifying Subtype Specific Drug Candidates International Journal of Molecular Sciences 10.3390/ijms262412031 2025
40538442 Integrated spatial omics of metabolic reprogramming and the tumor microenvironment in pancreatic cancer iScience 10.1016/j.isci.2025.112681 2025
40993391 SPP1 is required for maintaining mesenchymal cell fate in pancreatic cancer Nature 10.1038/s41586-025-09574-y 2025
41291066 RNF187 neddylation in pancreatic cancer activates malignancy via IQGAP1-dependent actin cytoskeleton rearrangement Oncogene 10.1038/s41388-025-03639-y 2025
39883522 Elevated protein lactylation promotes immunosuppressive microenvironment and therapeutic resistance in pancreatic ductal adenocarcinoma The Journal of Clinical Investigation 10.1172/JCI187024 2025
37697370 Combination of RUNX1 inhibitor and gemcitabine mitigates chemo‐resistance in pancreatic ductal adenocarcinoma by modulating BiP/PERK/eIF2α-axis-mediated endoplasmic reticulum stress Journal of Experimental & Clinical Cancer Research 10.1186/s13046-023-02814-x 2023
ADGRG6 Promotes Pancreatic Adenocarcinoma Progression Through the NF-κB/STAT6 Axis and Modulation of the Tumor Immune Microenvironment Current Issues in Molecular Biology 10.3390/cimb47120991 2025
41502505 Pan-Cancer Analysis of Enhancer-Induced PAN3-AS1 and Experimental Validation as a WFDC13-Promoting Factor in Colon Cancer Oncology Research 10.32604/or.2025.069274 2025
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 31625016
National Natural Science Foundation of China (NSFC) 81672443
National Natural Science Foundation of China (NSFC) 81773292
Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine 2017I2M1001
Ministry of Science and Technology of the People's Republic of China (MOST) National Key R&D Program of China 2018YFA0109700
Chinese Academy of Sciences (CAS) Strategic Priority Research Program of Chinese Academy of Sciences (CAS) XDA16010501
Submitter sun baofa (sunbf@big.ac.cn)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2018-10-12

Project Data

Resource name Description
BioSample (35)  show -
GSA (1) -
CRA001160 GSA-PDAC