| 项目编号 |
PRJCA003249 |
| 项目标题 |
Single-cell Transcriptomics Identifies Divergent Developmental Lineage Trajectories during Human Pituitary Development |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
The anterior pituitary gland plays central roles in body growth, reproduction, metabolism and the stress response. In this study, we performed single-cell RNA-sequencing (scRNA-seq) of 4,113 individual cells from human fetal pituitaries. We characterized divergent developmental trajectories with distinct transitional intermediate states in five hormone-producing cell lineages. Corticotropes exhibit an early intermediate state prior to full differentiation. Three cell types of the PIT-1 lineage (somatotropes, lactotropes and thyrotropes) segregate from a common progenitor coexpressing lineage-specific transcription factors of different sublineages. Gonadotropes experience two multistep developmental trajectories. Interestingly, a fetal gonadotrope cell subtype expressing the primate-specific hormone chorionic gonadotropin was identified. We also characterized the cellular heterogeneity of pituitary stem cells and identified a hybrid epithelial/mesenchymal state and an early-to-late state transition. Here, our results provide key insights into the transcriptional landscape of human pituitary development, defining distinct cell substates and subtypes and illustrating transcription factor dynamics during cell fate commitment. The gene expression matrix and cell information were deposited in ftp://download.big.ac.cn/gsa/CRA003091/other_files/. |
| 样品范围 |
Single cell |
| 发布日期 |
2020-09-16 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 33077725
|
Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development
|
Nature Communications
|
10.1038/s41467-020-19012-4
|
2020
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFC1003100
|
Research and development of new techniques for preimplantation genetic diagnosis
|
| National Natural Science Foundation of China (NSFC)
|
Major Research Plan
|
31871457
|
Single-cell transcriptome and epigenome analyses of human pituitary development
|
|
|
| 项目相关网站链接 |
|
| 提交者 |
Fuchou
Tang (tangfuchou@pku.edu.cn)
|
| 提交单位 |
Peking University |
| 提交日期 |
2020-08-14 |
