| 项目编号 |
PRJCA003631 |
| 项目标题 |
The hidden diversity of circular RNAs unveiled by Nanopore Sequencing |
| 涉及领域 |
Genetics |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
We propose a novel sequencing protocol for circular RNA enrichment and full-length sequencing of circular RNA isoforms using Nanopore sequencing. By utilizing circular reverse transcription and fragment size selection, circRNAs can be efficiently enriched (20 fold higher than previous methods) from total RNA. With the advantages of Nanopore sequencing, our method has promising applicability in unbiased characterization and reconstruction of full-length circRNA sequences. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2021-01-30 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
31722031
|
|
|
|
| 提交者 |
Zhang
Jinyang (zhangjinyang@biols.ac.cn)
|
| 提交单位 |
Beijing Institutes of Life Science |
| 提交日期 |
2020-10-09 |
