项目编号 PRJCA003807
项目标题 CRISPR screen
涉及领域 Medical
数据类型 Epigenomics
Raw sequence reads
Genome sequencing
物种名称 Mus musculus
Homo sapiens
描述信息 We developed a simple- and cost-effective controlled template-dependent elongation (CTDE) approach that can convert any DNA sample to a sgRNAs library. Then applying CRISPR-screening to genome-widely interrogate the function of individual epigenetic hallmarks demands massive sgRNA libraries which are unaffordable via commercial syntheses.
样品范围 Monoisolate
发布日期 2020-11-01
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
36938898 A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions Nucleic Acids Research 10.1093/nar/gkad198 2023
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
National Natural Science Foundation of China (NSFC) No.31771634
提交者 ran li (ranli1993@zju.edu.cn)
提交单位 Zhejiang University
提交日期 2020-11-01

项目包含数据信息

资源名称 描述
BioSample (726)  show -
GSA (1) -
CRA003465 Whole-genome epigenetic function annotation through sgRNA libraries synthesized by controlled template-dependent elongation