| Accession |
PRJCA004314 |
| Title |
Sleep loss reprograms the landscape of human immune cells toward inflammatory, aging and autoimmune phenotypes |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
As a naturally recurring state, sleep is key and indispensable to life. Although poor or insufficient sleep is an increasingly important public health issue, we know little about the cellular and molecular mechanisms by which sleep loss leads to immune dysfunction and link sleep to disease. Here, we combined scRNA-seq and mass cytometry to present a comprehensive and integrated single-cell landscape of human circulating immune cell in peripheral blood collected from pre- and post-24h sleep loss-subjects. We report that human subjected to 24h sleep loss had increased frequency of T cell, plasma B cell in blood, along with increased expression of autoimmunity-related markers and enriched pathways, indicative of autoreactive predisposition. In addition, the alterations of transcriptomics covering cytokines, chemokines, transcription factor and signal pathways, demonstrates sleep loss-induced accelerated systemic low-grade inflammation and cellular senescence. In independent validation cohorts, we confirmed that blood immune cells were activated and displayed evidence of enhanced cytokine-production profile in blood from participants after sleep loss. |
| Sample scope |
Single cell |
| Release date |
2021-01-20 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 34824394
|
Effects of poor sleep on the immune cell landscape as assessed by single-cell analysis
|
Communications Biology
|
10.1038/s42003-021-02859-8
|
2021
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2017YFA0105800
|
|
|
|
| Submitter |
Wenru
Su (suwr3@mail.sysu.edu.cn)
|
| Organization |
Zhongshan Ophthalmic Center,Sun Yat-sen University |
| Submission date |
2021-01-20 |
