| HRA000604
(Controlled Access)
|
Poor sleep is an important public health issue, but little is known about the cellular and molecular mechanisms by which sleep loss leads to immune dysfunction and disease. Here, we combined scRNA-seq and mass cytometry to obtain a comprehensive single-cell landscape of human circulating immune cells in blood from subjects pre- and post-24-h sleep loss. Participants subjected to 24-h sleep loss had increased T and plasma B cell frequencies, along with increased autoimmune-related marker expression and enriched pathways indicative of an autoreactive predisposition. Moreover, sleep loss-induced alterations in the transcriptome demonstrated accelerated systemic low-grade inflammation and cellular senescence. In validation cohorts, activated blood immune cells displayed enhanced cytokine-production profiles after sleep loss. Finally, we identified an expanded GM-CSF+ IFN-g+ T cell subset characterized by TNF-a and CCR2 co-production after sleep loss. |
