Accession

PRJCA004466

Title

Generation and Characterization of Stable Pig Pre-Gastrulation Epiblast Stem Cell Lines

Relevance

Agricultural

Data types

Whole genome sequencing
Epigenomics
Transcriptome or Gene expression
Raw sequence reads

Organisms

Sus scrofa

Description

single-cell RNA-sequencing profiling of porcine preimplantation embryos from embryonic day (E) 0 to E14, we successfully developed an in vitro culture system for establishing and maintaining stable pluripotent stem cell lines from porcine E10 pre-gastrulation epiblasts (termed pgEpiSCs).ultra-deep in situ Hi-C analysis of the pgEpiSCs and porcine embryonic fibroblast revealed functional impacts of 3D-spatial associations on the transcriptional regulation of pluripotency marker genes.

Sample scope

Monoisolate

Release date

2021-12-02

Publication

PubMed ID	Article title	Journal name	DOI	Year
34848870	Generation and characterization of stable pig pregastrulation epiblast stem cell lines	Cell Research	10.1038/s41422-021-00592-9	2022

Grants

Agency	program	Grant ID
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2018YFC1003100
Chinese Academy of Sciences (CAS)	Youth Innovation Promotion Association	2020104
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2019YFA0110700
Ministry of Science and Technology of the People's Republic of China (MOST)	International (Regional) Joint Research Programs	2020YFA0113200
Chinese Academy of Sciences (CAS)	General Program	2021081
National Natural Science Foundation of China (NSFC)	General Program	31970825
National Natural Science Foundation of China (NSFC)	General Program	31772601
National Natural Science Foundation of China (NSFC)	General Program	31601941
National Natural Science Foundation of China (NSFC)	General Program	31571497
Beijing Natural Science Foundation		6192005
Plan 111		B12008
Research Programs from the State Key Laboratories for Agro-biotechnology, China Agricultural University		2020SKLAB1-3
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2020YFA0509500
National Natural Science Foundation of China (NSFC)	International (Regional) Joint Research Programs	U19A2036
National Natural Science Foundation of China (NSFC)	General Program	31772576
the Science and Technology Program of Sichuan		2021YFYZ0009

Submitter

Caihong Zheng (zhengch@big.ac.cn)

Organization

Beijing Institute of Genomics, Chinese Academy of Sciences

Submission date

2021-02-07

Project Data

Resource name	Description
BioSample (3)	-
SAMC341726	pEFs
SAMC341725	pgEpiSCs
SAMC341724	pEmbryo
GSA (1)	-
CRA003960	Generation and characterization of stable porcine pre-gastrulation epiblast stem cell lines