| 项目编号 |
PRJCA005357 |
| 项目标题 |
single-cell RNA sequencing of colon cancer stem cell (CCSCs) xenograft tumor or low-notch activity tumor cell derived from asymmetric division of stem cells |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
For colon cancer stem cell xenograft tumor, single-cell RNA-seq were performed by the 10xGenomics platform.CD133+LGR5+ colon cancer stem cell(CCSCs) were subcutaneously injected into nude mice to form xenografts, which were collected and dissociated into single cells for single-cell RNA sequencing.For low-notch activity tumor cell derived from asymmetric division of stem cells, single-cell RNA-seq were performed by the SMART-seq2 platform.High-Notch-activity(GFP+) colon cancer stem cell(CCSCs) were sorted and seeded for dividing once, while low-Notch-activity daughter cells were collected from the dividing duplets for single-cell RNA-seq. |
| 样品范围 |
Synthetic |
| 发布日期 |
2023-06-01 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
|
|
2017YFA0504103
|
|
|
|
| 提交者 |
pengcheng
bu (bupc@ibp.ac.cn)
|
| 提交单位 |
Institute of Biophysics, Chinese Academy of Sciences |
| 提交日期 |
2021-06-02 |
