样本编号	SAMC1713749
外部数据库编号	GSA-Human: HRS180087
样品名称	CCSC xenograft tumor
样本标题	CCSC xenograft tumor
样品类型	Human sample
物种名称	Homo sapiens
描述信息	For colon cancer stem cell xenograft tumor, single-cell RNA-seq were performed by the 10xGenomics platform.CD133+LGR5+ CCSCs were subcutaneously injected into nude mice to form xenografts, which were collected and dissociated into single cells for single-cell RNA sequencing
样本属性	*该样本包含更多受控访问信息，请通过GSA-Human系统申请项目HRA000908数据获取。
发布日期	2023-06-01
项目编号	PRJCA005357
提交者	fei zhang (bupc@ibp.ac.cn)
提交单位	Institute of Biophysics, Chinese Academy of Sciences
提交日期	2023-06-18

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA000908 (Controlled Access)	For colon cancer stem cell xenograft tumor, single-cell RNA-seq were performed by the 10xGenomics platform.CD133+LGR5+ colon cancer stem cell(CCSCs) were subcutaneously injected into nude mice to form xenografts, which were collected and dissociated into single cells for single-cell RNA sequencing.For low-notch activity tumor cell derived from asymmetric division of stem cells, single-cell RNA-seq were performed by the SMART-seq2 platform.High-Notch-activity(GFP+) colon cancer stem cell(CCSCs) were sorted and seeded for dividing once, while low-Notch-activity daughter cells were collected from the dividing duplets for single-cell RNA-seq.

资源名称

描述

GSA-Human (1)

HRA000908 (Controlled Access)

For colon cancer stem cell xenograft tumor, single-cell RNA-seq were performed by the 10xGenomics platform.CD133+LGR5+ colon cancer stem cell(CCSCs) were subcutaneously injected into nude mice to form xenografts, which were collected and dissociated into single cells for single-cell RNA sequencing.For low-notch activity tumor cell derived from asymmetric division of stem cells, single-cell RNA-seq were performed by the SMART-seq2 platform.High-Notch-activity(GFP+) colon cancer stem cell(CCSCs) were sorted and seeded for dividing once, while low-Notch-activity daughter cells were collected from the dividing duplets for single-cell RNA-seq.