| 项目编号 |
PRJCA005636 |
| 项目标题 |
Deciphering the genetic basis of metabolome variation underlying maize drought response and tolerance |
| 涉及领域 |
Agricultural |
| 数据类型 |
Transcriptome or Gene expression
Metabolome
|
| 物种名称 |
Zea mays
|
| 描述信息 |
Metabolites are important regulators of plant stress responses. The genetic architecture of metabolome variation underlying plant drought response (DR) and tolerance (DT) remains elusive. Here, we performed non-targeted metabolic profiling of 385 maize natural inbred lines grown under well-watered (WW) and drought-stressed (DS) conditions. The 385 maize inbred lines were sown and grown in two growth pools at Huazhong Agricultural University, Wuhan, China. Each pool was divided into 400 plots with equal area, and 14 plants of each inbred line were grown in the plot. Maize plant in one pool were well-watered and the soil moisture was kept at around 25% and plants in the other pool were stop irrigation at 4-leave stage. Soil moisture were monitored by using a Delta-T HH2 soil moisture meter. Once the soil moisture of drought-treated pool reached 10%, the top fully expanded leaves of at least three plants (at around 6-leaf stage) were pooled and snap-frozen in liquid nitrogen and stored at -80°C for later metabolite extraction. 20mg freeze-dried powder of each sample was extracted followed the Methanol/MTBE protocol, and analyzed on an ACQUITY UPLC HSS T3 1.8 µm 2.1x100mm Waters Column on a Waters Acquity UPLC system coupled to a Thermo Fisher Q Extractive. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2021-07-16 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
International (Regional) Cooperation and Exchange Program
|
32061143031
|
Combine novel germplasms and multi-omics to mine maize drought-tolerant genes and use them in breeding
|
|
|
| 提交者 |
Mingqiu
Dai (mingqiudai@mail.hzau.edu.cn)
|
| 提交单位 |
Huazhong Agricultural University |
| 提交日期 |
2021-06-29 |
