| 项目编号 |
PRJCA005792 |
| 项目标题 |
Deciphering cell lineage specification of human lung adenocarcinoma with single-cell RNA sequencing |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Genome sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Lung adenocarcinomas (LUAD) arise from precancerous lesions such as atypical adenomatous hyperplasia, which progress into adenocarcinoma in situ and minimally invasive adenocarcinoma, then finally into invasive adenocarcinoma. The cellular heterogeneity and molecular events underlying this stepwise progression remain unclear. In this study, we performed single-cell RNA sequencing of 268,471 cells collected from 25 patients in four histologic stages of LUAD and compared them to normal epithelial cell types. We detected a group of cells closely resembling alveolar type 2 cells (AT2) that emerged during atypical adenomatous hyperplasia and whose transcriptional profile began to diverge from that of AT2 cells as LUAD progressed, taking on feature characteristic of stem-like cells. We identified several genes related to energy metabolism and ribosome synthesis that were upregulated in early stages of LUAD and may promote progression. MDK and TIMP1 emerged as potential prognostic biomarkers in LUAD. Stromal and immune cells in the lung tumor microenvironment may show tumor-promoting and immune-suppressing activities, making them key determinants of prognosis. Taken together, our data shed light on the underlying transcriptional signatures of distinct histologic stages of LUAD progression and may facilitate early diagnosis and prognostic prediction. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2021-07-09 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39043808
|
Multi-omics analysis unveils immunosuppressive microenvironment in the occurrence and development of multiple pulmonary lung cancers
|
NPJ Precision Oncology
|
10.1038/s41698-024-00651-5
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81871890
|
|
|
|
| 提交者 |
Li
Weimin (weimi003@scu.edu.cn)
|
| 提交单位 |
Frontiers Science Center for Disease-related Molecular Networks, West China Hospital, Sichuan University, Chengdu, Sichuan, China |
| 提交日期 |
2021-07-09 |
