| Accession |
PRJCA005792 |
| Title |
Deciphering cell lineage specification of human lung adenocarcinoma with single-cell RNA sequencing |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
Genome sequencing
|
| Organisms |
Homo sapiens
|
| Description |
Lung adenocarcinomas (LUAD) arise from precancerous lesions such as atypical adenomatous hyperplasia, which progress into adenocarcinoma in situ and minimally invasive adenocarcinoma, then finally into invasive adenocarcinoma. The cellular heterogeneity and molecular events underlying this stepwise progression remain unclear. In this study, we performed single-cell RNA sequencing of 268,471 cells collected from 25 patients in four histologic stages of LUAD and compared them to normal epithelial cell types. We detected a group of cells closely resembling alveolar type 2 cells (AT2) that emerged during atypical adenomatous hyperplasia and whose transcriptional profile began to diverge from that of AT2 cells as LUAD progressed, taking on feature characteristic of stem-like cells. We identified several genes related to energy metabolism and ribosome synthesis that were upregulated in early stages of LUAD and may promote progression. MDK and TIMP1 emerged as potential prognostic biomarkers in LUAD. Stromal and immune cells in the lung tumor microenvironment may show tumor-promoting and immune-suppressing activities, making them key determinants of prognosis. Taken together, our data shed light on the underlying transcriptional signatures of distinct histologic stages of LUAD progression and may facilitate early diagnosis and prognostic prediction. |
| Sample scope |
Multiisolate |
| Release date |
2021-07-09 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
81871890
|
|
|
|
| Submitter |
Li
Weimin (weimi003@scu.edu.cn)
|
| Organization |
Frontiers Science Center for Disease-related Molecular Networks, West China Hospital, Sichuan University, Chengdu, Sichuan, China |
| Submission date |
2021-07-09 |
