| HRA001130
(Controlled Access)
|
In this study, we performed single-cell RNA sequencing of 268,471 cells collected from 25 patients in four histologic stages of LUAD and compared them to normal epithelial cell types. We detected a group of cells closely resembling alveolar type 2 cells (AT2) that emerged during atypical adenomatous hyperplasia and whose transcriptional profile began to diverge from that of AT2 cells as LUAD progressed, taking on feature characteristic of stem-like cells. We identified genes related to energy metabolism and ribosome synthesis that were upregulated in early stages of LUAD and may promote progression. MDK and TIMP1 emerged as potential prognostic biomarkers in LUAD. Taken together, our data shed light on the underlying transcriptional signatures of distinct histologic stages of LUAD progression and may facilitate early diagnosis and prognostic prediction. |
