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Accession PRJCA005957
Title Single-cell multiomics sequencing analysis of spindle-transferred human embryos
Relevance Medical
Data types Whole genome sequencing
Epigenomics
Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
Organisms Homo sapiens
Description Mitochondrial DNA (mtDNA) mutations are often associated with incurable diseases and lead to live birth defects in 1 out of 200 babies. Uncoupling of the inheritance of mtDNA and the nuclear genome by spindle transfer (ST) can potentially prevent the transmission of mtDNA mutations from mother to offspring. However, no well-established studies have critically assessed the safety of this technique. Here, using single-cell triple omics sequencing method, this project is aimed to systematically analyze the genome (copy number variation), DNA methylome, and transcriptome of ST and control blastocysts. This study has a profound impact on deciding whether to proceed with the clinical trial of ST.
Sample scope Single cell
Release date 2021-07-27
Publication
PubMed ID Article title Journal name DOI Year
35972936 Single-cell multiomics analyses of spindle-transferred human embryos suggest a mostly normal embryonic development PLoS Biology 10.1371/journal.pbio.3001741 2022
37766497 Single-cell multi-omics sequencing reveals chromosome copy number inconsistency between trophectoderm and inner cell mass in human reconstituted embryos after spindle transfer Human Reproduction 10.1093/humrep/dead186 2023
Grants
Agency program Grant ID Grant title
the Beijing Municipal Science and Technology Commission Z181100001318001
Ministry of Science and Technology of the People's Republic of China (MOST) 2018YFC1003003
Submitter Fuchou    Tang  (tangfuchou@pku.edu.cn)
Organization Peking University
Submission date 2021-07-27

Project Data

Resource name Description
BioSample (46)  show -