| HRA001110
(Open Access)
|
Mitochondrial DNA (mtDNA) mutations are often associated with incurable diseases and lead to live birth defects in 1 out of 200 babies. Uncoupling of the inheritance of mtDNA and the nuclear genome by spindle transfer (ST) can potentially prevent the transmission of mtDNA mutations from mother to offspring. However, no well-established studies have critically assessed the safety of this technique. Here, using single-cell triple omics sequencing method, this project is aimed to systematically analyze the genome (copy number variation), DNA methylome, and transcriptome of ST and control blastocysts. This study has a profound impact on deciding whether to proceed with the clinical trial of ST. |
