| Accession |
PRJCA005972 |
| Title |
Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages |
| Relevance |
Medical |
| Data types |
Epigenomics
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
Spinal ependymomas are the most common spinal cord tumors in adults, but their intratumoral cellular heterogeneity has been less studied, and how spinal microglia are involved in tumor progression is still unknown. Here, our single-cell RNA-sequencing analyses of three spinal ependymoma subtypes dissected the microenvironmental landscape of spinal ependymomas and revealed tumor-associated macrophage (TAM) subsets with distinct functional phenotypes. CCL2+ TAMs were related to the immune response and exhibited a high capacity for apoptosis, while CD44+ TAMs were associated with tumor angiogenesis. By combining these results with those of scATAC-seq data analysis, we revealed that TEAD1 and EGR3 play roles in regulating the functional diversity of TAMs. We further identified diverse characteristics of both malignant cells and TAMs that might underlie the different malignant degrees of each subtype. Finally, assessment of cell-cell interactions revealed that stromal cells act as extracellular factors that mediate TAM diversity. Overall, our results revealed dual functions of TAMs in tumor progression, providing valuable insights for TAM-targeting immunotherapy. |
| Sample scope |
Single cell |
| Release date |
2024-03-27 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 34824203
|
Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages
|
Nature Communications
|
10.1038/s41467-021-27018-9
|
2021
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Chinese Academy of Sciences (CAS)
|
|
XDA16020601
|
Strategic Priority Research Program of the Chinese Academy of Sciences
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2019YFA0110100
|
|
|
|
| Submitter |
Xiaoqun
Wang (xiaoqunwang@ibp.ac.cn)
|
| Organization |
Institute of Biophysics, Chinese Academy of Sciences |
| Submission date |
2021-07-28 |
