| 项目编号 |
PRJCA005972 |
| 项目标题 |
Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages |
| 涉及领域 |
Medical |
| 数据类型 |
Epigenomics
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Spinal ependymomas are the most common spinal cord tumors in adults, but their intratumoral cellular heterogeneity has been less studied, and how spinal microglia are involved in tumor progression is still unknown. Here, our single-cell RNA-sequencing analyses of three spinal ependymoma subtypes dissected the microenvironmental landscape of spinal ependymomas and revealed tumor-associated macrophage (TAM) subsets with distinct functional phenotypes. CCL2+ TAMs were related to the immune response and exhibited a high capacity for apoptosis, while CD44+ TAMs were associated with tumor angiogenesis. By combining these results with those of scATAC-seq data analysis, we revealed that TEAD1 and EGR3 play roles in regulating the functional diversity of TAMs. We further identified diverse characteristics of both malignant cells and TAMs that might underlie the different malignant degrees of each subtype. Finally, assessment of cell-cell interactions revealed that stromal cells act as extracellular factors that mediate TAM diversity. Overall, our results revealed dual functions of TAMs in tumor progression, providing valuable insights for TAM-targeting immunotherapy. |
| 样品范围 |
Single cell |
| 发布日期 |
2024-03-27 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 34824203
|
Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages
|
Nature Communications
|
10.1038/s41467-021-27018-9
|
2021
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Chinese Academy of Sciences (CAS)
|
|
XDA16020601
|
Strategic Priority Research Program of the Chinese Academy of Sciences
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2019YFA0110100
|
|
|
|
| 提交者 |
Xiaoqun
Wang (xiaoqunwang@ibp.ac.cn)
|
| 提交单位 |
Institute of Biophysics, Chinese Academy of Sciences |
| 提交日期 |
2021-07-28 |
