| 项目编号 | PRJCA006674 | ||||||||||||||||||||||||||||
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| 项目标题 | Two waves of spatiotemporal H3K9me3 allocations collectively organize human zygotic genome activation and differentiation initiation | ||||||||||||||||||||||||||||
| 涉及领域 | Model organism | ||||||||||||||||||||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Genome sequencing |
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| 物种名称 |
Mus musculus
Homo sapiens |
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| 描述信息 | Trimethylated histone H3 lysine 9 (H3K9me3) modification regulates the genomic transcriptional activity and cell fate transition. However, the reprogramming dynamics and the underlying molecular mechanisms of H3K9me3 during the pre-implantation development of human embryos remain completely elusive. Here we profiled high-resolution genome-wide H3K9me3 in human oocytes and pre-implantation embryos. Conserved in mouse, H3K9me3 is significantly enriched in long terminal repeats (LTRs) and is progressively established along with human embryogenesis. Moreover, after zygotic genome activation (ZGA), human embryos also exhibit epigenetic switch from DNA methylation to H3K9me3 to silence temporarily activated retrotransposons. Notably, we discovered two spatial-temporal waves of H3K9me3 deposition at peri-ZGA and post-ZGA, which mediate the silence of distinct LTR families. DUX is identified as the potential transcription factor regulating human peri-ZGA-specific H3K9me3 allocation, while typical H3K9me3-related co-regulators are responsible for the establishment of post-ZGA-specific H3K9me3. Finally, we found that strong bivalency of H3K4me3/H3K9me3 and H3K4me4/H3K27me3 modifications exist in human blastocysts, as nascent priming for lineage differentiation. Taken together, our results provide insights into the dynamic pattern of H3K9me3-dependent silencing of LTRs during human early development and largely improve our understanding of the regulatory network during human maternal-to-zygotic transition and the first lineage specification. | ||||||||||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||||||||||
| 发布日期 | 2025-02-06 | ||||||||||||||||||||||||||||
| 项目资金来源 |
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| 提交者 | Xiaoyu Liu (liuxiaoyu@tongji.edu.cn) | ||||||||||||||||||||||||||||
| 提交单位 | Tongji University | ||||||||||||||||||||||||||||
| 提交日期 | 2021-09-24 |