样本编号	SAMC1912211
外部数据库编号	GSA-Human: HRS302238
样品名称	Human_ICM_Smart-seq_rep1
样本标题	Human ICM Smart-seq rep1
样品类型	Human sample
物种名称	Homo sapiens
描述信息	Two waves of spatiotemporal H3K9me3 allocations collectively organize human zygotic genome activation and differentiation initiation
样本属性	*由于样本关联的数据集HRA001391尚未在科技部完成人类遗传资源信息备案，本页面只展示部分样本信息。
发布日期	2022-07-28
项目编号	PRJCA006674
提交者	QIU WU (liuxiaoyu@tongji.edu.cn)
提交单位	Tongji University
提交日期	2023-06-18

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA001391 (Open Access)	We profiled high-resolution genome-wide H3K9me3 in human oocytes and pre-implantation embryos. Conserved in mouse, H3K9me3 is significantly enriched in long terminal repeats (LTRs) and is progressively established along with human embryogenesis. Moreover, after zygotic genome activation (ZGA), human embryos also exhibit epigenetic switch from DNA methylation to H3K9me3 to silence temporarily activated retrotransposons. Notably, we discovered two spatial-temporal waves of H3K9me3 deposition at peri-ZGA and post-ZGA, which mediate the silence of distinct LTR families. DUX is identified as the potential transcription factor regulating human peri-ZGA-specific H3K9me3 allocation, while typical H3K9me3-related co-regulators are responsible for the establishment of post-ZGA-specific H3K9me3. Finally, we found that strong bivalency of H3K4me3/H3K9me3 and H3K4me4/H3K27me3 modifications exist in human blastocysts, as nascent priming for lineage differentiation.

资源名称

描述

GSA-Human (1)

HRA001391 (Open Access)

We profiled high-resolution genome-wide H3K9me3 in human oocytes and pre-implantation embryos. Conserved in mouse, H3K9me3 is significantly enriched in long terminal repeats (LTRs) and is progressively established along with human embryogenesis. Moreover, after zygotic genome activation (ZGA), human embryos also exhibit epigenetic switch from DNA methylation to H3K9me3 to silence temporarily activated retrotransposons. Notably, we discovered two spatial-temporal waves of H3K9me3 deposition at peri-ZGA and post-ZGA, which mediate the silence of distinct LTR families. DUX is identified as the potential transcription factor regulating human peri-ZGA-specific H3K9me3 allocation, while typical H3K9me3-related co-regulators are responsible for the establishment of post-ZGA-specific H3K9me3. Finally, we found that strong bivalency of H3K4me3/H3K9me3 and H3K4me4/H3K27me3 modifications exist in human blastocysts, as nascent priming for lineage differentiation.