| Description |
Lentinula edodes is a tetrapolar basidiomycete, which is popular cultivated in East Asia. Difference with plants and animals, L. edodes are performance in heterokaryons with two haploid nuclei in most of their life cycle, rather than diploids. Therefore, it is particularly important to analyze the difference between the two haploid nuclear genomes to understand the role of the two nuclei in the entire life cycle of L. edodes. In this study, we assembled two mating dedikaryotic monokaryons SP3 and SP30 by with HiFi and Hi-C technology. Finally, we assembly total length of 50.93 Mb SP3 and 49.80 Mb SP30 genome in 10 chromosomes with anchoring rate of about 99.63% and 98.91% for contigs more than 100 Kb, respectively. According to comparative genomic analysis, the chromosome lengths of the two nuclei are significant different, and there is not a complete correspondence between the chromosomes. Collinearity analysis shows the proportion of special genes in both nuclei is close to 30%, and according to enrichment the two nuclei exhibited different functions in carbohydrate metabolism, secondary metabolism and spliceosome formation. SP30 special genes were significant enriched on starch and sucrose metabolism and easier to use the substrate in PDA medium to grow. The growth phenotypic of the two monokaryons have proved this inference. Additionally, the two mating type loci of L. edodes are located on two different chromosomes. At more than 2 Mb far from HD locus, we annotated a new incomplete HD sublocus with HD2 domain on both monokaryons. In addition to HD locus, two highly homologous rcb and phb genes were also found in both monokaryons. This study lays a foundation for further revealing allelic differences and synergistic effect of two nuclei in fruiting body formation, and will enlighten us to further study the evolution and variation of cultivated L. edodes. |
