| Accession |
PRJCA008650 |
| Title |
Long-term functional maintenance of primary human hepatocytes in vitro |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
Raw sequence reads
|
| Organisms |
Homo sapiens
|
| Description |
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening. |
| Sample scope |
Multiisolate |
| Release date |
2023-12-14 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 31023926
|
|
|
|
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2017YFA0103000
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2016YFA0100103
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31730059
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31521004
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81670590
|
|
|
|
| Submitter |
Hongkui
Deng (hongkui_deng@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2022-03-14 |
