样本编号 SAMC2066839
外部数据库编号 GSA-Human: HRS362842
样品名称 DMSO_D3_B2
样本标题 DMSO_D3_B2
样品类型 Human sample
物种名称 Homo sapiens
描述信息 DMSO treated human hepatocytes
样本属性 *由于样本关联的数据集HRA002121尚未在科技部完成人类遗传资源信息备案,本页面只展示部分样本信息。
发布日期 2023-12-14
项目编号 PRJCA008650
提交者 Jinlin  Wang  (hongkui_deng@pku.edu.cn)
提交单位 Peking University
提交日期 2023-06-18

样本包含数据信息

资源名称 描述
GSA-Human (1) -
HRA002121  (Open Access) The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.