Accession PRJCA011582
Title Two enhancers synergistically regulate HoxB genes expressions and retinoic acid-induced early ESCs differentiation through 3D chromatin structure
Relevance Medical
Data types Raw sequence reads
Organisms Mus musculus
Description Homeobox B cluster (HoxB) genes play important roles in RA-induced early ESCs differentiation. In this study, we identified two enhancers synergistically regulate HoxB genes expressions through 3D chromatin structure and thus regulate RA-induced early ESCs differentiation. 4C data showed interactions between HoxB genes and the two enhancers. CRISPR/Cas9-mediated individual or compound deletion of the two enhancers significantly inhibits HoxB genes expressions while transcriptome analysis revealed that RA induced early ESCs differentiation was blocked in the enhancer KO cells. Our study suggests new mechanism by which two enhancers regulate HoxB genes expressions and retinoic acid-induced early ESCs differentiation through 3D chromatin structure.
Sample scope Multiisolate
Release date 2022-08-31
Publication
PubMed ID Article title Journal name DOI Year
Two Enhancers Regulate HoxB Genes Expression During Retinoic Acid-Induced Early Embryonic Stem Cells Differentiation Through Long-Range Chromatin Interactions Stem Cells and Development 10.1089/scd.2021.0020 2021
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2017YFA0102600
Accessions in other database
Accession Database name
SRP303688 NCBI
Submitter Wenbin Wang (sciencedb@gibh.ac.cn)
Organization Nankai University
Submission date 2022-08-31

Project Data

Resource name Description
BioSample (22)  show -
GSA (1) -
CRA008027 Two enhancers synergistically regulate HoxB genes expressions and retinoic acid-induced early ESCs differentiation through 3D chromatin structure