| 项目编号 |
PRJCA014442 |
| 项目标题 |
In-organoid single-cell CRISPR screening reveals determinants of hepatocyte differentiation and maturation |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Harnessing differentiated and mature hepatocytes for basic research and regenerative medicine demands understanding its underlying principles. Despite tremendous efforts that have been made to generate functional hepatocytes from stem cells, the immature phenotype necessitates deeper investigations of the genetic circuit of differentiation and maturation. Single-cell CRISPR (scCRISPR) screens could link genetic perturbations with parallel transcriptomic readout in single cells, providing a powerful method to delineate multiple genes' contributions to a complex biological process. Here, we have developed a method named Organoid-based Single-cell CRISPR screening Analyzed with Regulons (OSCAR) to functionally assess candidate genes regulating hepatocyte differentiation and maturation. |
| 样品范围 |
Single cell |
| 发布日期 |
2023-02-03 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 37907970
|
In-organoid single-cell CRISPR screening reveals determinants of hepatocyte differentiation and maturation
|
Genome Biology
|
10.1186/s13059-023-03084-8
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key Research and Development Program of China
|
|
2018YFA0109800
|
|
| National Key Research and Development Program of China
|
|
2018YFA0109400
|
|
| National Natural Science Foundation of China
|
|
32122023
|
|
| National Natural Science Foundation of China
|
|
32022022
|
|
| National Natural Science Foundation of China
|
|
82172887
|
|
| Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences
|
|
CIFMS2021I2M1066
|
|
| State Key Laboratory Special Fund
|
|
2060204
|
|
|
|
| 提交者 |
Jun
Cao (caojundudu@qq.com)
|
| 提交单位 |
Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College |
| 提交日期 |
2023-01-14 |
