项目编号

PRJCA016400

项目标题

Single-cell reconstruction of developmental trajectories across amphioxus embryogenesis reveals neural system regionalization along the anterior-posterior axis

涉及领域

Evolution

数据类型

Epigenomics

物种名称

Branchiostoma floridae

描述信息

Amphioxus is an invertebrate chordate that is phylogenetically well positioned for advancing our understanding of vertebrate evolution. Here we report the development of single-cell RNA-sequencing (scRNA-seq) data from seven amphioxus embryo stages from the blastula to late neurula stages. We also deployed scRNA-seq in an experimental framework to compare Brachyury double knock-out and wild type embryos. This demonstrated that Brachyury is essential for balancing posterior neural and mesodermal cell fate decisions and we provide evidence for the presence of neuromesodermal progenitor cells in amphioxus. We suggest that the evolution of these in a stem chordate led to a dramatic shift in posterior neural and mesodermal cell fate decisions and the body elongation that is part of the chordate body plan.

样品范围

Single cell

发布日期

2024-06-28

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
National Natural Science Foundation of China (NSFC)		32070458
National Natural Science Foundation of China (NSFC)		32061160471
National Natural Science Foundation of China (NSFC)		32070815
National Natural Science Foundation of China (NSFC)		32270439
National Natural Science Foundation of China (NSFC)		32100335
National Natural Science Foundation of China (NSFC)		82050002
Office of China Postdoc Council 2021 Postdoctoral International Exchange Program, Talent-Introduction Program Postdoc		273565
Institute of Zoology Chinese Academy of Sciences Bingzhi Postdoctoral Fellowship Program		00000001

提交者

Xuming Zhou (zhouxuming@ioz.ac.cn)

提交单位

Institute of Zoology, Chinese Academy of Sciences

提交日期

2023-04-19

项目包含数据信息

资源名称	描述
BioSample (24) show	-
SAMC1215987	T1 stage, replicate 5, scRNA-seq
SAMC1215986	T1 stage, replicate 4, scRNA-seq
SAMC1215985	T1 stage, replicate 3, scRNA-seq
SAMC1215984	T1 stage, replicate 2, scRNA-seq
SAMC1215983	T1 stage, replicate 1, scRNA-seq
SAMC1215982	N4 stage, replicate 4, scRNA-seq
SAMC1215981	N4 stage, replicate 3, scRNA-seq
SAMC1215980	N4 stage, replicate 2, scRNA-seq
SAMC1215979	N4 stage, replicate 1, scRNA-seq
SAMC1215978	N2 stage, knockout, replicate 2, scRNA-seq
SAMC1215977	N2 stage, knockout, replicate 1, scRNA-seq
SAMC1215976	N2 stage, control, replicate 2, scRNA-seq
SAMC1215975	N2 stage, control, replicate 1, scRNA-seq
SAMC1215974	N0 stage, replicate 4, scRNA-seq
SAMC1215973	N0 stage, replicate 3, scRNA-seq
SAMC1215972	N0 stage, replicate 2, scRNA-seq
SAMC1215971	N0 stage, replicate 1, scRNA-seq
SAMC1215970	G4 stage, replicate 2, scRNA-seq
SAMC1215969	G4 stage, replicate 1, scRNA-seq
SAMC1215968	G0 stage, replicate 3, scRNA-seq
SAMC1215967	G0 stage, replicate 2, scRNA-seq
SAMC1215966	G0 stage, replicate 1, scRNA-seq
SAMC1215965	B stage, replicate 2, scRNA-seq
SAMC1215964	B stage, replicate 1, scRNA-seq
GSA (1)	-
CRA010773	Single-cell reconstruction of developmental trajectories across amphioxus embryogenesis reveals neural system regionalization along the anterior-posterior axis