| 样本编号 |
SAMC1215971 |
| 样品名称 |
N0_stage_sample_1 |
| 样本标题 |
N0 stage, replicate 1, scRNA-seq |
| 样品类型 |
Model organism or animal sample |
| 物种名称 |
Branchiostoma floridae |
| 描述信息 |
Amphioxus is an invertebrate chordate that is phylogenetically well positioned for advancing our understanding of vertebrate evolution. Here we report the development of single-cell RNA-sequencing (scRNA-seq) data from seven amphioxus embryo stages from the blastula to late neurula stages. We also deployed scRNA-seq in an experimental framework to compare Brachyury double knock-out and wild type embryos. This demonstrated that Brachyury is essential for balancing posterior neural and mesodermal cell fate decisions and we provide evidence for the presence of neuromesodermal progenitor cells in amphioxus. We suggest that the evolution of these in a stem chordate led to a dramatic shift in posterior neural and mesodermal cell fate decisions and the body elongation that is part of the chordate body plan. |
| 样本属性 |
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品种
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种
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年龄
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实验材料提供者
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Institute of zoology, CAS, Xuming Zhou
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性别
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not applicable
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组织器官
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Embryo
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出生时间
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出生地点
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品种历史
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饲养方法
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细胞系
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Whole embryo
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细胞亚型
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细胞类型
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采样单位
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收集/保存方法
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死亡日期
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发育阶段
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疾病名称
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疾病分期
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基因型
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Wildtype
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地理分布
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养殖方法
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健康状况
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取样来源
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经纬度
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表型
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类型
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凭证标本
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贮藏条件
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记录册编号
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处理方法
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中医症候
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| 发布日期 |
2024-06-28 |
| 项目编号 |
PRJCA016400 |
| 提交者 |
Xuming Zhou (weiqiang_liu2015@outlook.com)
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| 提交单位 |
Institute of Zoology, Chinese Academy of Sciences |
| 提交日期 |
2023-04-24 |
