| Accession | PRJCA017452 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq | ||||||||||
| Relevance | Medical | ||||||||||
| Data types | Single cell sequencing | ||||||||||
| Organisms |
Acinetobacter baumannii
Escherichia coli Pseudomonas aeruginosa Klebsiella pneumoniae Staphylococcus aureus |
||||||||||
| Description | we report a droplet-based high-throughput single-microbe RNA-seq assay (smRandom-seq), using random primers for in situ cDNA generation, droplets for single microbe barcoding, and CRISPR-based rRNA depletion for mRNA enrichment. | ||||||||||
| Sample scope | Multispecies | ||||||||||
| Release date | 2023-06-30 | ||||||||||
| Publication |
|
||||||||||
| Grants |
|
||||||||||
| Submitter | Yongcheng Wang (yongcheng@zju.edu.cn) | ||||||||||
| Organization | First Affiliated Hospital, Zhejiang University | ||||||||||
| Submission date | 2023-06-03 |
| Resource name | Description |
|---|---|
| BioSample (5) | - |
| SAMC1275472 | Sau |
| SAMC1275471 | Pae |
| SAMC1275470 | Kpn |
| SAMC1275469 | Aba |
| SAMC1275468 | Eco |
| GSA (1) | - |
| CRA011274 | Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq |