| Accession | PRJCA022405 | ||||||||||||||||
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| Title | METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity | ||||||||||||||||
| Relevance | Medical | ||||||||||||||||
| Data types |
Transcriptome or Gene expression
Single cell sequencing ribo-seq |
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| Organisms |
Mus musculus
Homo sapiens |
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| Description | Upon activation, B cells exit their quiescent state and enter germinal center responses with massive changes in translation. Here, we identify METTL1 as a translational checkpoint during B cell activation. Conditional knock out of METTL1 in B cells blocks GC entry and impairs GC formation, leading to accumulation of GC precursors. In contrast, conditional knock-in of METTL promotes GC responses. Mechanistically, METTL catalyzes mG modification on specific subset of transfer RNAs that leads to biased preferential translation in B cell receptor signaling pathway. BCR signaling promotes electron transporter chain activities to ensure sufficient bioenergy. Pathologically, METTL mediated tRNA mG modification promotes B cell differentiation in SLE and deletion of METTL alleviates auto-reactive B cell responses during autoimmune induction. Overall, our study uncovers the essential role of METTL for effective B cell responses and aberrant tRNA mG modification by METTL promotes auto-reactive B cells in systemic autoimmunity. | ||||||||||||||||
| Sample scope | Single cell | ||||||||||||||||
| Release date | 2024-10-25 | ||||||||||||||||
| Grants |
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| Submitter | Hui Zhang (zhangh656@mail.sysu.edu.cn) | ||||||||||||||||
| Organization | The First Affiliated Hospital, Sun Yat-sen University | ||||||||||||||||
| Submission date | 2023-12-26 |
| Resource name | Description |
|---|---|
| BioSample (17) show | - |
| GSA (4) | - |
| CRA014155 | METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity |
| CRA018131 | TRAC-seq data of METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity |
| CRA017868 | bulk rna-seq data of METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity |
| CRA014160 | ribo-seq and rna-seq data of METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity |