样本编号	SAMC4048551
外部数据库编号	GSA-Human: HRS1329434
样品名称	vehicle
样本标题	vehicle
样品类型	Human sample
物种名称	Homo sapiens
描述信息	293T cells were transfected with an empty vector control plasmid. At 48 hours post-transfection, cells were subjected to UV crosslinking at 4000*100 µJ/cm2 energy. Cells were then lysed, and anti-FLAG magnetic beads were used for immunoprecipitation of crosslinked RNA-protein complexes for 4 hours at 4°C with rotation. This is the input sample.
样本属性	*该样本包含更多受控访问信息，请通过GSA-Human系统申请项目HRA008135数据获取。
发布日期	2024-10-25
项目编号	PRJCA022405
提交者	Hui Zhang (zhangh656@mail.sysu.edu.cn)
提交单位	The First Affiliated Hospital, Sun Yat-sen University
提交日期	2024-08-01

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA008135 (Controlled Access)	293T cells were transfected with either an exogenous METTL1 overexpression plasmid or an empty vector control plasmid. At 48 hours post-transfection, cells were subjected to UV crosslinking at 4000*100 µJ/cm2 energy. Cells were then lysed, and anti-FLAG magnetic beads were used for immunoprecipitation of crosslinked RNA-protein complexes for 4 hours at 4°C with rotation. RNA was subsequently extracted from the immunoprecipitated material using TRIzol reagent following the manufacturer's instructions. Recombinant wild-type AlkB and the D135S AlkB mutant proteins were employed to remove dominant methylations from the isolated RNA samples, facilitating efficient reverse transcription of tRNAs.

资源名称

描述

GSA-Human (1)

HRA008135 (Controlled Access)

293T cells were transfected with either an exogenous METTL1 overexpression plasmid or an empty vector control plasmid. At 48 hours post-transfection, cells were subjected to UV crosslinking at 4000*100 µJ/cm2 energy. Cells were then lysed, and anti-FLAG magnetic beads were used for immunoprecipitation of crosslinked RNA-protein complexes for 4 hours at 4°C with rotation. RNA was subsequently extracted from the immunoprecipitated material using TRIzol reagent following the manufacturer's instructions. Recombinant wild-type AlkB and the D135S AlkB mutant proteins were employed to remove dominant methylations from the isolated RNA samples, facilitating efficient reverse transcription of tRNAs.