| Accession | PRJCA029459 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title | NEAT-seq: A NSR Primed and Transposase Tagmentation Mediated Strand-specific Total RNA Sequencing in Single Cell | ||||||||
| Relevance | Model organism | ||||||||
| Data types | Transcriptome or Gene expression | ||||||||
| Organisms |
Mus musculus
Homo sapiens |
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| Description | Single-cell RNA sequencing (scRNA-seq) has transformed our understanding of cellular diversity with unprecedented resolution. However, many current methods are limited in capturing full-length transcripts and discerning strand orientation. We present NEAT-seq, an innovative strand-specific total RNA sequencing technique that combines not-so-random (NSR) primers with Tn5 transposase-mediated tagmentation. NEAT-seq overcomes previous limitations by delivering comprehensive transcript coverage and maintaining strand orientation, which is essential for accurate quantification of overlapping genes and detection of antisense transcripts. Through optimized reverse transcription with NSR primers, rRNA depletion via DASH (Depletion of Abundant Sequences by Hybridization), and linear amplification, NEAT-seq enhances sensitivity and reproducibility, especially for low-input samples and single cells. Application to mouse oocytes and early embryos highlights NEAT-seq's superior performance in identifying stage-specific antisense transcripts, shedding light on their regulatory roles during early development. This advancement represents a significant leap in transcriptome analysis within complex biological contexts. | ||||||||
| Sample scope | Monoisolate | ||||||||
| Release date | 2024-10-04 | ||||||||
| Grants |
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| Submitter | Yang Yu (yulabngs@163.com) | ||||||||
| Organization | Institute of Biophysics, Chinese Academy of Sciences | ||||||||
| Submission date | 2024-08-25 |