| Accession | SAMC4104261 |
|---|---|
| Accession in Other Database | GSA-Human: HRS1342837 |
| Sample name | RNAseq_HEK293_10ng_DASH_rep1 |
| Title | RNAseq_HEK293_10ng_DASH_rep1 |
| Sample type | Human sample |
| Organism | Homo sapiens |
| Description | NEATseq of 10ng total RNA of HEK293 cells with DASH treatment, using Maxima H Minus reverse transcriptase, NSR primers |
| Attributes | *This sample record contains additional controlled-access information that is avaiable from GSA-Human by requesting study HRA008391 in the GSA-Human system. |
| Release date | 2024-10-04 |
| BioProject Accession | PRJCA029459 |
| Submitter | Ming Wang (yulabngs@163.com) |
| Organization | Institute of biophysics, Chinese Academy of Sciences |
| Submission date | 2024-08-27 |
| Resource name | Description |
|---|---|
| GSA-Human (1) | - |
| HRA008391 (Controlled Access) | We present NEAT-seq, an innovative strand-specific total RNA sequencing technique that combines not-so-random (NSR) primers with Tn5 transposase-mediated tagmentation. NEAT-seq overcomes previous limitations by delivering comprehensive transcript coverage and maintaining strand orientation, which is essential for accurate quantification of overlapping genes and detection of antisense transcripts. Through optimized reverse transcription with NSR primers, rRNA depletion via DASH (Depletion of Abundant Sequences by Hybridization), and linear amplification, NEAT-seq enhances sensitivity and reproducibility, especially for low-input samples and single cells. |