| 项目编号 |
PRJCA032741 |
| 项目标题 |
ALCAM is an Entry Factor for Severe Community Acquired Pneumonia-associated Human Adenovirus Species B |
| 涉及领域 |
Medical |
| 数据类型 |
Raw sequence reads
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Human adenovirus (HAdV) is one of the most widely spread respiratory pathogens that can cause infections in multiple tissues and organs. Previous studies have established an association between HAdV species B (HAdV-B) infection and severe community-acquired pneumonia (SCAP). However, the connection between SCAP-associated HAdV-B infection and host factor expression profile in patients has not been systematically investigated. Here, we performed CRISPR genetic screen on HAdV-B using two generations of cell surface protein-focused CRISPR libraries and identified a series of host factors including the known receptor DSG-2 and an unknown factor activated leukocyte cell adhesion molecule (ALCAM). Further investigation has shown that ALCAM affects HAdV-B infection by participating in viral internalization. Most importantly, re-visit of the clinical samples has uncovered that ALCAM has significantly higher expression in SCAP patients with HAdV-B infection than in those with other infections. Chimeric and authentic virus experiments have suggested that ALCAM is a widely used host factor across B1 and B2 genetic clusters of HAdV-B. Moreover, the dissociation constant between the knob domain of HAdV-B fiber and ALCAM was determined to be 837 nM in average. In summary, our results suggest that ALCAM is a functional entry factor for SCAP-associated HAdV-B. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-11-24 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39738070
|
ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B
|
Nature Communications
|
10.1038/s41467-024-55261-3
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2023ZD0506200
|
Research on the prevention and treatment of cancer, cardiovascular and cerebrovascular diseases, respiratory diseases, and metabolic diseases
|
|
|
| 提交者 |
Jieming
Qu (jmqu0906@163.com)
|
| 提交单位 |
Ruijin Hospital Institute of Respiratory Diseases, School of Medicine, Shanghai Jiao-Tong University |
| 提交日期 |
2024-11-24 |
