| HRA009475
(Controlled Access)
|
Human adenovirus (HAdV) is one of the most widely spread respiratory pathogens that can cause infections in multiple tissues and organs. Previous studies have established an association between HAdV species B (HAdV-B) infection and severe community-acquired pneumonia (SCAP). However, the connection between SCAP-associated HAdV-B infection and host factor expression profile in patients has not been systematically investigated. Here, we performed CRISPR genetic screen on HAdV-B using two generations of cell surface protein-focused CRISPR libraries and identified a series of host factors including the known receptor DSG-2 and an unknown factor activated leukocyte cell adhesion molecule (ALCAM). Further investigation has shown that ALCAM affects HAdV-B infection by participating in viral internalization. Most importantly, re-visit of the clinical samples has uncovered that ALCAM has significantly higher expression in SCAP patients with HAdV-B infection than in those with other infections. |
