| HRA001318
(Controlled Access)
|
Human papillomavirus (HPV) is an important cause of cervical cancer. HPV encoded oncogenes E6 and E7 lead to a large number of genomic structural mutations through interference WITH DNA repair, which is directly related to the occurrence and development of cervical cancer. The second generation of whole genome sequencing, currently widely used in HPV detection, is difficult to accurately analyze integration sites and structural variations. Nanopore third-generation sequencing (ONT), with a single-read length of 1Mb, can make up for the deficiency of second-generation sequencing. We will further analyze and verify whether HPV genomic insertion affects the expression of genes at the insertion site and the expression of related pathway proteins regulated by these genes. This study will fill the gap in the accurate diagnosis of cervical cancer by third-generation sequencing, and is expected to provide a new analytical platform for the accurate treatment of cervical cancer. |
