| Accession | SAMC1989373 | ||||||||||||||||||||||||||||||||||||||||||
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| Accession in Other Database | GSA-Human: HRS321240 | ||||||||||||||||||||||||||||||||||||||||||
| Sample name | sc5mC_K562_4 | ||||||||||||||||||||||||||||||||||||||||||
| Title | Cabernet sequencing of K562: single-cell | ||||||||||||||||||||||||||||||||||||||||||
| Sample type | Human sample | ||||||||||||||||||||||||||||||||||||||||||
| Organism | Homo sapiens | ||||||||||||||||||||||||||||||||||||||||||
| Description | Validation of high-coverage bisulfite-free single-cell methylome sequencing method | ||||||||||||||||||||||||||||||||||||||||||
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| Release date | 2023-12-23 | ||||||||||||||||||||||||||||||||||||||||||
| BioProject Accession | PRJCA007640 | ||||||||||||||||||||||||||||||||||||||||||
| Submitter | Tianjiao Yuan (yunlongcao@pku.edu.cn) | ||||||||||||||||||||||||||||||||||||||||||
| Organization | Peking University | ||||||||||||||||||||||||||||||||||||||||||
| Submission date | 2023-06-18 |
| Resource name | Description |
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| GSA-Human (1) | - |
| HRA001740 (Open Access) | DNA methylation, as a crucial epigenetic modification, plays an essential role in genome regulation. Dysregulation of DNA methylation usually means disruption of development and disease.Here, we developed carrier-assisted base-conversion by enzymatic reaction with end-tagging (Cabernet) technology, which enables bisulfite-free detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at single-cell level and single-base resolution. Cabernet can provide a high-detectability method for 5mC, 5hmC and hemi-methylation profiling at single-cell level. |