| 项目编号 | PRJCA007640 | ||||||||
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| 项目标题 | A High Coverage Bisulfite-free Single-cell Methylome Sequencing Method | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 |
Epigenomics
Raw sequence reads Single cell sequencing |
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| 物种名称 |
Mus musculus
Homo sapiens |
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| 描述信息 | DNA methylation, as a crucial epigenetic modification, plays an essential role in genome regulation. Dysregulation of DNA methylation usually means disruption of development and disease. The emergence of bisulfite sequencing provides tool to identify cell type-specific DNA methylation differences. Bisulfite sequencing is considered the gold standard for DNA methylation measurement. However, bisulfite treatment presents a pretty harsh chemical condition and causes intense DNA degradation, which limits the genomic coverage in single-cell scenario. Here, we developed carrier-assisted base-conversion by enzymatic reaction with end-tagging (Cabernet) technology, which enables bisulfite-free detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at single-cell level and single-base resolution. In the meanwhile, Cabernet can detect DNA hemi-methylation with capability of allele assessment and distinguishment of Watson and Crick strands. We performed Cabernet for mouse early embryos to accurately profile 5mC, 5hmC and hemi-methylation dynamics. High-coverage single-cell methylome sequencing data sets allow precise lineage trancing of embryo development. Therefore, Cabernet can provide a high-detectability method for 5mC, 5hmC and hemi-methylation profiling at single-cell level. The landscape of DNA methylation reprogramming may give insights for understanding the mechanism of epigenetic regulation during preimplantation development. | ||||||||
| 样品范围 | Single cell | ||||||||
| 发布日期 | 2023-11-14 | ||||||||
| 项目资金来源 |
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| 提交者 | Yunlong Cao (yunlongcao@pku.edu.cn) | ||||||||
| 提交单位 | Peking University | ||||||||
| 提交日期 | 2021-12-14 |