Accession | SAMC1989523 | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Accession in Other Database | GSA-Human: HRS782738 | ||||||||||||||||||||||||||||||||||||||||||
Sample name | K562-96_L4.4_3 | ||||||||||||||||||||||||||||||||||||||||||
Title | sciCabernet sequencing of K562 | ||||||||||||||||||||||||||||||||||||||||||
Sample type | Human sample | ||||||||||||||||||||||||||||||||||||||||||
Organism | Homo sapiens | ||||||||||||||||||||||||||||||||||||||||||
Description | Validation of high-throughput bisulfite-free single-cell methylome sequencing method | ||||||||||||||||||||||||||||||||||||||||||
Attributes |
|
||||||||||||||||||||||||||||||||||||||||||
Custom attributes |
|
||||||||||||||||||||||||||||||||||||||||||
Release date | 2023-12-27 | ||||||||||||||||||||||||||||||||||||||||||
BioProject Accession | PRJCA007640 | ||||||||||||||||||||||||||||||||||||||||||
Submitter | Tianjiao Yuan (yunlongcao@pku.edu.cn) | ||||||||||||||||||||||||||||||||||||||||||
Organization | Peking University | ||||||||||||||||||||||||||||||||||||||||||
Submission date | 2023-06-18 |
Resource name | Description |
---|---|
GSA-Human (1) | - |
HRA001740 (Open Access) | DNA methylation, as a crucial epigenetic modification, plays an essential role in genome regulation. Dysregulation of DNA methylation usually means disruption of development and disease.Here, we developed carrier-assisted base-conversion by enzymatic reaction with end-tagging (Cabernet) technology, which enables bisulfite-free detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at single-cell level and single-base resolution. Cabernet can provide a high-detectability method for 5mC, 5hmC and hemi-methylation profiling at single-cell level. |