样本编号

SAMC1989788

外部数据库编号

样品名称

K562-96_L4.3_6

样本标题

sciCabernet sequencing of K562

样品类型

Human sample

物种名称

Homo sapiens

描述信息

Validation of high-throughput bisulfite-free single-cell methylome sequencing method

样本属性

分离株名称	not collected
年龄
实验材料提供者	BIOPIC
性别	female
组织器官	K-562 cell
疾病名称
细胞系
细胞亚型
细胞类型
收集/保存方法
发育阶段
疾病分期
血统性（民族）
健康状况
核型
表型
人口
种族（人种）
类型
处理方法
采样日期	2021-02-26

用户自定义属性

发布日期

2023-12-27

项目编号

PRJCA007640

提交者

Tianjiao Yuan (yunlongcao@pku.edu.cn)

提交单位

Peking University

提交日期

2023-06-18

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA001740 (Open Access)	DNA methylation, as a crucial epigenetic modification, plays an essential role in genome regulation. Dysregulation of DNA methylation usually means disruption of development and disease.Here, we developed carrier-assisted base-conversion by enzymatic reaction with end-tagging (Cabernet) technology, which enables bisulfite-free detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at single-cell level and single-base resolution. Cabernet can provide a high-detectability method for 5mC, 5hmC and hemi-methylation profiling at single-cell level.

资源名称

描述

GSA-Human (1)

HRA001740 (Open Access)

DNA methylation, as a crucial epigenetic modification, plays an essential role in genome regulation. Dysregulation of DNA methylation usually means disruption of development and disease.Here, we developed carrier-assisted base-conversion by enzymatic reaction with end-tagging (Cabernet) technology, which enables bisulfite-free detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at single-cell level and single-base resolution. Cabernet can provide a high-detectability method for 5mC, 5hmC and hemi-methylation profiling at single-cell level.