| HRA002777
(Controlled Access)
|
T cell activation is a key event in adaptive immunity. However, the effects of cell status, cell heterogeneity and cellular communication on T cell activation have not been systematically analyzed. We found resting CD4 T cells differentiated into conventional effector T cells (conv TEFF), cytokines high TEFF (cytohi TEFF), proliferation TEFF (prolif TEFF) and heat shock proteins (HSPs) high expressed T (HSPhi T) upon anti-CD3/CD28 stimulation. We called the stimulated T cells overlapped with resting T on UMAP as inert T. Different from resting T, inert T showed intermediate feature of T cell activation including expression of cytokines and activated T specific marker genes. Resting CD4 T cells were more likely to differentiate into cytohi TEFF and were less likely to differentiate into HSPhi T and Th0 in the presence of CD8 T cells. Resting CD4 T cells respond to anti-CD3/CD28 differently from either CD4 TN or CD4 TM, due to difference of cell states, cell-cell communications and specific microenvironment. |
