| HRA005140
(Controlled Access)
|
To identify novel host factors that enable SARS-CoV-2 infection, we performed a genome-wide CRISPRa screen in HEK293T cells. After two rounds of infection by SARS-CoV-2 pseudovirus with an EGFP marker, the library cells were sorted for total EGFP+ cells as well as the top 10%-20%, top 10% and top 2% grouped by the EGFP intensity. The reference sample and each group of EGFP+ cells were subjected to genomic extraction, PCR amplification of the sgRNAeBAR sequences and high-throughput sequencing. |
