Libraries were generated by double-stranded (DS) protocol. We identified and retained the cytosine-deaminated terminal sequences seen in typical aDNA and constructed a uracil-DNA glycosylase ("UDG") partially-treated double-stranded (denoted as "DS_half") library. The in-solution capture was accomplished by overlapping probes with DNA fragments and enriching the resulting libraries. The probes were synthesized based on the human mitochondrial genome.
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QT_T0602M42
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Institute of cultural relics and archaeology in Zhengzhou City