样本编号	SAMC3547110
外部数据库编号	GSA-Human: HRS1225796
样品名称	M2 RNA-seq
样本标题	M2 RNA-seq
样品类型	Human sample
物种名称	Homo sapiens
描述信息	M2 RNA-seq
样本属性	*由于样本关联的数据集HRA007223尚未在科技部完成人类遗传资源信息备案，本页面只展示部分样本信息。
发布日期	2024-04-23
项目编号	PRJCA024613
提交者	Zhang Long (l_zhang@zju.edu.cn)
提交单位	Life Sciences Institute, Zhejiang University
提交日期	2024-04-23

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA007223 (Open Access)	PBMC were isolated via Lymphocyte Separation Media (Multi Science, 70-LSM01) following the protocol. Cells were washed twice using cold PBS and total RNA was extracted with the RNeasy micro kit (QIAGEN) according to the manual. RNA sequencing was performed with a Truseq RNA library prep kit v2 (Illumina) and illumina NovaSeq 6000 equipment. We used a perlscript to filter out low quality reads and subsequently the clean reads were mapped to human genome hg19 with TopHat2. Gene expression levels were calculated and normalized to fragments per kilobase of transcript per million mapped reads (FPKM).

资源名称

描述

GSA-Human (1)

HRA007223 (Open Access)

PBMC were isolated via Lymphocyte Separation Media (Multi Science, 70-LSM01) following the protocol. Cells were washed twice using cold PBS and total RNA was extracted with the RNeasy micro kit (QIAGEN) according to the manual. RNA sequencing was performed with a Truseq RNA library prep kit v2 (Illumina) and illumina NovaSeq 6000 equipment. We used a perlscript to filter out low quality reads and subsequently the clean reads were mapped to human genome hg19 with TopHat2. Gene expression levels were calculated and normalized to fragments per kilobase of transcript per million mapped reads (FPKM).