| HRA012284
(Open Access)
|
We developed and applied two high-throughput techniques, SNP-STARR-seq and Methyl-STARR-seq, to systematically evaluate the influence of 30,790 non-coding SNPs and over 134,000 CpG sites on enhancer activity in cells sourced from primary and metastatic colorectal cancer. Additionally, we generated two independent knockout HCT116 cell lines using CRISPR/Cas9, each with a short fragment (89 bp and 136 bp, respectively) spanning the target SNP rs6061231 removed from the genome. |
