Database Commons

a catalog of biological databases

e.g., animal; RNA; Methylation; China

Database Profile

General information

URL: http://primerdepot.nci.nih.gov/
Full name: qPrimerDepot
Description: qPrimerDepot is a primer database for quantitative real time PCR
Year founded: 2007
Last update: 2007-06-25
Version: v1.0
Accessibility:
Manual:
Accessible
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Country/Region: United States
Data type:
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Contact information

University/Institution: National Cancer Institute
Address: MD 20892-4605
City: Bethesda
Province/State: MD
Country/Region: United States
Contact name (PI/Team): Kevin Gardner
Contact email (PI/Helpdesk): gardnerk@mail.nih.gov

Publications

17068075
qPrimerDepot: a primer database for quantitative real time PCR. [PMID: 17068075]
Cui W, Taub DD, Gardner K.

Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

Nucleic Acids Res. 2007:35(Database issue) | 113 Citations (from Europe PMC, 2021-06-19)

Ranking

All databases:
1010/5061 (80.063%)
Expression:
182/924 (80.411%)
1010
Total Rank
113
Citations
8.071
z-index

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Record metadata

Created on: 2015-08-10
Curated by:
Lina Ma [2016-04-11]
Lin Xia [2016-03-28]