| URL: | http://bioinfo.life.hust.edu.cn/miR_path |
| Full name: | Tumor-miRNA-pathway |
| Description: | This is a database with user-friendly web interface to display the miRNA pathway regulation and their expressions in the 20 tumor types. The database provides search, browse and download function for the the miR-pathway data. Users can search miRNA regulating pathways, miRNA target genes and the expression profiles of miRNAs and genes in TCGA. |
| Year founded: | 2016 |
| Last update: | |
| Version: | |
| Accessibility: |
Accessible
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| Country/Region: | China |
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| University/Institution: | Huazhong University of Science and Technology |
| Address: | Hubei Bioinformatics and Molecular Imaging Key Laboratory, Department of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China |
| City: | Wuhan |
| Province/State: | Hubei |
| Country/Region: | China |
| Contact name (PI/Team): | Zhaowu Ma |
| Contact email (PI/Helpdesk): | mazw@hust.edu.cn |
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MicroRNA regulatory pathway analysis identifies miR-142-5p as a negative regulator of TGF-β pathway via targeting SMAD3. [PMID: 27683030]
MicroRNAs (miRNAs) are non-coding RNAs with functions of posttranscriptional regulation. The abnormally expressed miRNAs have been shown to be crucial contributors and may serve as biomarkers in many diseases. However, determining the biological function of miRNAs is an ongoing challenge. By combining miRNA targets prediction, miRNA and mRNA expression profiles in TCGA cancers, and pathway data, we performed a miRNA-pathway regulation inference by Fisher's exact test for enrichment analysis. Then we constructed a database to show the cancer related miRNA-pathway regulatory network (http://bioinfo.life.hust.edu.cn/miR_path). As one of the miRNAs targeting many cancer related pathways, miR-142-5p potentially regulates the maximum number of genes in TGF-? signaling pathway. We experimentally confirmed that miR-142-5p directly targeted and suppressed SMAD3, a key component in TGF-? signaling. Ectopic overexpression of miR-142-5p significantly promoted tumor cell proliferation and inhibited apoptosis, while silencing of miR-142-5p inhibited the tumor cell proliferation and promoted apoptosis in vitro. These findings indicate that miR-142-5p plays as a negative regulator in TGF-? pathway by targeting SMAD3 and suppresses TGF-?-induced growth inhibition in cancer cells. Our study proved the feasibility of miRNA regulatory pathway analysis and shed light on combining bioinformatics with experiments in the research of complex diseases. |