| URL: | https://www.peptracker.com/accounts/login/epd/ |
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| Description: | The Encyclopedia of Proteome Dynamics (EPD) provides unrestricted, public access to a large volume of proteomics data generated by the Lamond Laboratory and their collaborators. An account is not required to access these data.. However, the EPD also contains data from numerous experiments that are still in progress and where the analyses are not yet complete. Access to these data sets is restricted to PepTracker account holders only. |
| Year founded: | 2014 |
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Accessible
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| Country/Region: | United Kingdom |
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| University/Institution: | University of Dundee |
| Address: | Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, United States |
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| Country/Region: | United Kingdom |
| Contact name (PI/Team): | Angus I Lamond |
| Contact email (PI/Helpdesk): | a.i.lamond@dundee.ac.uk |
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A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells. [PMID: 24596151]
Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization procedures. We identify myeloid-specific gene expression and variations in protein abundance, isoform expression and phosphorylation at different cell cycle stages. We dissect the relationship between protein and mRNA levels for both bulk gene expression and for over ?6000 genes individually across the cell cycle, revealing complex, gene-specific patterns. This data set, one of the deepest surveys to date of gene expression in human cells, is presented in an online, searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/). DOI: http://dx.doi.org/10.7554/eLife.01630.001. |