| URL: | http://tomancak-srv1.mpi-cbg.de/DOT/main |
| Full name: | Dresden Ovary Table |
| Description: | The DOT presents visual 3D microscopy-based information about RNA localization for more then 1700 genes in the fruit fly adult ovary at all time-points of oogenesis. Altogether, the database contains more then 8000 in situ experiments documenting almost 7000 genes with 32,000 image stacks and over 50,000 exemplary 2D images. |
| Year founded: | 2015 |
| Last update: | |
| Version: | |
| Accessibility: |
Accessible
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| Country/Region: | Germany |
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| University/Institution: | Max Planck Institute of Molecular Cell Biology and Genetics |
| Address: | Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany |
| City: | Dresden |
| Province/State: | |
| Country/Region: | Germany |
| Contact name (PI/Team): | Pavel Tomancak |
| Contact email (PI/Helpdesk): | tomancak@mpi-cbg.de |
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Systematic imaging reveals features and changing localization of mRNAs in Drosophila development. [PMID: 25838129]
mRNA localization is critical for eukaryotic cells and affects numerous transcripts, yet how cells regulate distribution of many mRNAs to their subcellular destinations is still unknown. We combined transcriptomics and systematic imaging to determine the tissue-specific expression and subcellular distribution of 5862 mRNAs during Drosophila oogenesis. mRNA localization is widespread in the ovary and detectable in all of its cell types-the somatic epithelial, the nurse cells, and the oocyte. Genes defined by a common RNA localization share distinct gene features and differ in expression level, 3'UTR length and sequence conservation from unlocalized mRNAs. Comparison of mRNA localizations in different contexts revealed that localization of individual mRNAs changes over time in the oocyte and between ovarian and embryonic cell types. This genome scale image-based resource (Dresden Ovary Table, DOT, http://tomancak-srv1.mpi-cbg.de/DOT/main.html) enables the transition from mechanistic dissection of singular mRNA localization events towards global understanding of how mRNAs transcribed in the nucleus distribute in cells. |