| URL: | http://dpsc.ccbr.utoronto.ca/cancer/ |
| Full name: | Donnelly - Princess Margaret Screening Centre Cancer Database |
| Description: | The DPSC-Cancer database is a collection of shRNA dropout signatures profiles, covering ~16000 human genes, and derived from more than 70 Pancreatic, Ovarian and Breast human cancer cell-lines using the microarray detection platform developed in the DPSC (Donnelly - Princess Margaret Screening Centre) facility at the Moffat Lab. |
| Year founded: | 2012 |
| Last update: | NA |
| Version: | v1.0 |
| Accessibility: |
Accessible
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| Country/Region: | Canada |
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| University/Institution: | University of Toronto |
| Address: | University of Toronto,160 College St.,Toronto,ON M5S 3E1,Canada |
| City: | Toronto |
| Province/State: | ON |
| Country/Region: | Canada |
| Contact name (PI/Team): | Jason Moffat |
| Contact email (PI/Helpdesk): | j.moffat@utoronto.ca |
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COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines. [PMID: 22102578]
Genome-wide pooled shRNA screens enable global identification of the genes essential for cancer cell survival and proliferation and provide a 'functional genetic' map of human cancer to complement genomic studies. Using a lentiviral shRNA library targeting approximately 16,000 human genes and a newly developed scoring approach, we identified essential gene profiles in more than 70 breast, pancreatic and ovarian cancer cell lines. We developed a web-accessible database system for capturing information from each step in our standardized screening pipeline and a gene-centric search tool for exploring shRNA activities within a given cell line or across multiple cell lines. The database consists of a laboratory information and management system for tracking each step of a pooled shRNA screen as well as a web interface for querying and visualization of shRNA and gene-level performance across multiple cancer cell lines. COLT-Cancer Version 1.0 is currently accessible at http://colt.ccbr.utoronto.ca/cancer. |