生长方案:	-
处理方案:	-
提取方案:	To collect single blastomeres, embryos with good quality were transferred into 0.5% Pronase in TL-HEPES to remove zona pellucida, followed by three washes in PVP-BSA. To distinguish the outer and inner cells, embryos were incubated with PKH2 dye (Sigma) which labels the live outer cells with red fluorescence. After labeling, embryos were rinsed with Ca2+/Mg2+ free PVP-PBS and treated with 0.125% trypsin-EDTA for at least 5 min at 37℃ until the cell surface looks rough. Disaggregating was done by a mouth pipette aided by several finely pulled glass capillaries of different internal diameter. After most cells were separated apart, single cells were individually transferred into small droplets of PVP-PBS and the inner/outer cell identity was identified by the fluorescent microscopy. Cells showing no fluorescence were inner cells, whereas those with red fluorescence were outer cells.
建库方案:	Briefly, single cells were washed three times in BSA-PBS and those with a clear round shape were added into cell lysis buffer, followed by First-strand cDNA synthesis and PCR amplification. PCR products were purified by Ampure XP beads and used for libraries construction.Libraries were deep sequenced on Illumina X-ten platform, using 150-bp paired-end strategy.

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2500
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度