PRJNA435554: RNA sequencing of B cell subsets (CD11c hi IgD+ B cells, CD11c hi IgD- B cells, Memory B cells and Naïve B cells) from healthy subjects and subjects with Systemic lupus erythematosus (SLE) or Rheumatoid arthritis (RA)
概要: CD11c+ B cells (IgD+ and IgD-) are pathogenic B cells expanded in autoimmune disease. The purpose of this study is to identify the pathways unique to IgD+ CD11c B cells and IgD- CD11c B cells.
项目整体设计: B cell subsets were isolated from peripheral blood and RNA sequencing was performed with Hiseq 2000 platform
Peripheral blood B cells from SLE patients, healthy subjects or RA patients were isolated using human B cell enrichment kit (StemCell Technologies) and stained for indicated markers. The purity of the sorted populations was routinely >90%.
处理方案:
-
提取方案:
RNA was isolated using PicoPure RNA isolation kit (Thermo Fisher Scientific)
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2000
链特异性:
Unspecific
样本
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疾病
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文章
IL-21 drives expansion and plasma cell differentiation of autoreactive CD11chiT-bet+ B cells in SLE.
