Glioma is a complex malignant neoplasm, treating each case as a dark box obviously could not acquire expected overall prognosis, because subpopulation inside tumor could be core factor which directly influence tumor progression. So, clonal sequencing or single-cell sequencing could be best solution to this problem. Here we combined these two strategies and analyzed more than 6000 single-cell transcriptome of 73 surgery points from 13 glioma and 1 brain metastasis patient. After these cells asigned into normal, immune, tumor and several other groups based on differentially expressed genes and specified TCGA 4 classification gene sets, spacial information of sampling points helped us built up cell type constituent of different part inside tumor, and illustrated invasion patterns during tumor growth.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.